Effects and Mechanism of Resistin-like Molecules α on EndothelialCell Angiogenesis in Mouse Aorta

Abstract

Objective To explore the effects and mechanism of resistin-like molecules α(also known as FIZZ1) on the ability of mouse aortic endothelial cells to form new blood vessels. Methods Mouse was stimulated by FIZZ1 at 10 nmol/L,20 nmol/L and 40 nmol/L.The role of FIZZ1 in endothelial cell migration and blood vessel formation was verified by using endothelial cell tube formation experiments and endothelial cell migration experiments in vitro;The effect of FIZZ1on signaling in mouse aortic endothelial cells was detected by high-throughput sequencing,and pathways with significant differential expression signaling were selected. Results Under different concentrations of FIZZ1,the area of blood vessel formation in mouse aortic endothelial cells gradually increased,and 40 nmol/L FIZZ1/ RELMα had the strongest effect in promoting angiogenesis.With the increasing concentration of FIZZ1,the number of endothelial cells migrated increased,and the migration capacity gradually increased.A total of 1 253 genes were found to be differentially expressed,including 427 up-regulated genes and 826 down-regulated genes.The mechanism may be related to the significant expression difference of COL1A1,FLT 4 and ITGA 3. Conclusion FIZZ1 can induce neovascularization of mouse aortic endothelial cells in vitro,and its mechanism may be related to significant differences in gene expression such as COL1A1,FLT4,and ITGA3.

Keywords

• resistin-like molecules α
• angiogenesis
• endothelial cells
• aorta
• mice