Extracellular vesicle miRNAs from three-dimensional ovarian cancer in vitro models and their implication in overall cancer survival
Nihar Godbole,
Andrew Lai,
Flavio Carrion,
Katherin Scholz-Romero,
Akhilandeshwari Ravichandran,
Priyakshi Kalita-de Croft,
Amy E. McCart Reed,
Vaibhavi Joshi,
Sunil R. Lakhani,
Mostafa Kamal Masud,
Yusuke Yamauchi,
Lewis Perrin,
John Hooper,
Laura Bray,
Dominic Guanzon,
Carlos Salomon
Affiliations
Nihar Godbole
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Andrew Lai
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Flavio Carrion
Departamento de Investigación, Postgrado y Educación Continua (DIPEC), Facultad de Ciencias de la Salud, Universidad del Alba, Santiago 8320000, Chile
Katherin Scholz-Romero
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Akhilandeshwari Ravichandran
Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD 4059, Australia; School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane City, QLD 4000, Australia; ARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology, Kelvin Grove, QLD 5059, Australia
Priyakshi Kalita-de Croft
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Amy E. McCart Reed
UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Vaibhavi Joshi
UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia
Sunil R. Lakhani
UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Pathology Queensland, The Royal Brisbane and Women's Hospital, Herston, QLD 4029, Australia
Mostafa Kamal Masud
UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD 4072, Australia
Yusuke Yamauchi
Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD 4072, Australia
Lewis Perrin
UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Mater Research Institute, Translational Research Institute, Woolloongabba, QLD 4102, Australia
John Hooper
UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Mater Research Institute, Translational Research Institute, Woolloongabba, QLD 4102, Australia
Laura Bray
Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD 4059, Australia; School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane City, QLD 4000, Australia; ARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology, Kelvin Grove, QLD 5059, Australia
Dominic Guanzon
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Corresponding author. Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group Faculty of Health Sciences, University of Queensland, Royal Brisbane Hospital, Building 71/918, Herston, QLD 4029, Australia.
Carlos Salomon
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia; Corresponding author. UQ Centre for Extracellular Vesicle Nanomedicine Head, Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, UQ Centre for Clinical Research, Australia.
Ovarian cancer is the most common gynaecological malignancy and the seventh most diagnosed cancer in females worldwide. Currently, it is the sixth leading cause of cancer related mortality among patients globally. The heterogenous origin of the disease and unambiguous nature of the clinical symptoms leading to delayed detection has been one of the key reasons for increasing mortality. Hence new approaches are required to understand the biology of ovarian cancer, where the use of cell culture models that mimic the physiology of the disease is fundamental. Cell culture serves as a crucial in vitro tool, contributing to our comprehension of various aspects of cell biology, tissue morphology, disease mechanisms, drug responses, protein production, and tissue engineering. A significant portion of in vitro studies rely on two-dimensional (2D) cell cultures, however, these cultures present notable limitations, for example disruptions in cellular and extracellular interactions, alterations in cell morphology, polarity, and division mechanisms. Recently, extracellular vesicles have been identified as crucial players in cell biology as part of the communication system that cancer cells use to metastasize. We optimized and compared three-dimensional (3D) culture of ovarian cancer cells lines (SKOV-3 and OVCAR-3) with two-dimensional models based on their protein and miRNA content. We further investigated whether extracellular vesicles from these models reflect changes in cancer cells, and aid in the identification of overall survival in women with ovarian cancer.
