Stem Cell Research (Mar 2016)

Generation of KCL033 clinical grade human embryonic stem cell line

  • Liani Devito,
  • Anastasia Petrova,
  • Victoria Wood,
  • Neli Kadeva,
  • Glenda Cornwell,
  • Stefano Codognotto,
  • Emma Stephenson,
  • Dusko Ilic

DOI
https://doi.org/10.1016/j.scr.2015.12.047
Journal volume & issue
Vol. 16, no. 2
pp. 296 – 299

Abstract

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The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.