Isolation of extended‐spectrum β‐lactamase‐producing Escherichia coli from Japanese red fox (Vulpes vulpes japonica)

Abstract

Abstract Antimicrobial resistance is a global concern requiring a one‐health approach. Given wild animals can harbor antimicrobial‐resistant bacteria (ARB), we investigated their presence in 11 fecal samples from wild animals using deoxycholate hydrogen sulfide lactose agar with or without cefotaxime (CTX, 1 mg/L). Thus, we isolated CTX‐resistant Escherichia coli from two Japanese red fox fecal samples. One strain was O83:H42‐ST1485‐fimH58 CTX‐M‐55‐producing E. coli carrying the genes aph(3″)‐Ib, aph(3′)‐Ia, aph(6)‐Id, mdf(A), sitABCD, sul2, tet(A), and tet(B), whereas the other was O25:H4‐ST131‐fimH30 CTX‐M‐14‐producing E. coli carrying mdf(A) and sitABCD and showing fluoroquinolone resistance. Thus, the presence of extended‐spectrum β‐lactamase producers in wild foxes suggests a spillover of ARB from human activities to these wild animals.

Keywords

• antimicrobial resistance
• ESBL
• fox
• wildlife