Diosmetin protects against retinal injury via reduction of DNA damage and oxidative stress

Zeren Shen; Jinjin Shao; Jiabin Dai; Yuchen Lin; Xiaochun Yang; Jian Ma; Qiaojun He; Bo Yang; Ke Yao; Peihua Luo

Toxicology Reports (Jan 2016)

Diosmetin protects against retinal injury via reduction of DNA damage and oxidative stress

Zeren Shen,
Jinjin Shao,
Jiabin Dai,
Yuchen Lin,
Xiaochun Yang,
Jian Ma,
Qiaojun He,
Bo Yang,
Ke Yao,
Peihua Luo

Affiliations

Zeren Shen: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China; Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China
Jinjin Shao: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China
Jiabin Dai: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China
Yuchen Lin: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China; Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China
Xiaochun Yang: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China
Jian Ma: Center for Drug Safety Evaluation and Research of Zhejiang University, Hangzhou, P.R. China
Qiaojun He: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China
Bo Yang: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China
Ke Yao: Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China
Peihua Luo: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China; Corresponding author at: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang, P.R. China. Fax: +86 571 88208400.

Journal volume & issue: Vol. 3
pp. 78 – 86

Abstract

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Visual impairment is a global public health problem that needs new candidate drugs. Chrysanthemum is a traditional Chinese drug, famous for its eye-protective function, with an unclear mechanism of action. To determine how chrysanthemum contributes to vision, we identified, for the first time, the component of chrysanthemum, diosmetin (DIO), which acts in protecting the injured retina in an adriamycin (ADR) improving model. We observed that DIO could attenuate the apoptosis of retinal cells in Sprague–Dawley rats and verified this effect in cultured human retinal pigment epithelium (RPE) cells, ARPE-19. Our further study on the mechanism revealed the counteractive effect of DIO on the attenuation of DNA damage and oxidative stress, which occurs in a wide range of retinal disorders. These results collectively promise the potential value of DIO as a retinal-protective agent for disorders that lead to blindness. In addition, we identified, for the first time, the component of chrysanthemum, DIO, which acts in protecting the injured retina. Keywords: Apoptosis, Chrysanthemum, Diosmetin, DNA damage, Oxidative stress, Retinal injury, Retinal pigment epithelium

Published in Toxicology Reports

ISSN: 2214-7500 (Online)
Publisher: Elsevier
Country of publisher: Ireland
LCC subjects: Medicine: Public aspects of medicine: Toxicology. Poisons
Website: http://www.journals.elsevier.com/toxicology-reports/

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