Prognostic value of circ_0000043/miR-590-5p in cervical cancer and regulation of tumor progression

Abstract

Abstract Objective To investigate the prognostic value of circ_0000043 in cervical cancer (CC) and its involvement in the modulation of patients’ prognostic progression. Methods Clinical data of 136 CC patients were included, postoperative CC and adjacent normal tissues were stored at −80 °C, and patients were followed up for 5 years for prognosis. RT-qPCR detected circ_ 0000043 and miR-590-5p expression, Kaplan–Meier curves recorded for prognostic survival, multivariate cox analysis of patients’ prognostic risk factors. Moreover, CCK8 assessed cell proliferation, Transwell detected migration and invasion. Dual luciferase reporter assay and RIP examined the interactions between circ_ 0000043 and miR-590-5p. Results circ_ 0000043 was down-regulated in CC patients, and patients with low expression of circ_ 0000043 had worse survival. Low expression of circ_0000043, lymph node metastasis, and FIGO were all unfavorable factors threatening the prognosis of CC. circ_0000043 overexpression markedly inhibited the proliferation, migration, and invasion of cancer cells. miR-590-5p is a direct target miRNA of circ_0000043. miR-590-5p mimic prominently resisted the altered cellular functions induced by circ_0000043. Conclusion Patients with low circ_0000043 expression have poor prognosis as it fuels CC progression by boosting cancer cell proliferation, migration, and invasion via miR-590-5p. Our study provides new ideas for the prognosis and treatment of CC.

Keywords

• Circ_ 00000431
• Cervical cancer
• MiR-590-5p
• Prognosis