MOLECULAR GENETIC ANALYSIS OF INFECTION AGENTS OF FARM ANIMALS ANAPLASMOSIS ON THE TERRITORY OF WESTERN AND EASTERN SIBERIA

V. A. Par; T. I. Yepikhina; E. A. Yefremova; V. A. Marchenko; O. V. Suntsova; O. V. Lisak; E. K. Doroshchenko; I. M. Zubareva; A. Y. Tikunov; N. V. Tikunova

Acta Biomedica Scientifica (Sep 2015)

MOLECULAR GENETIC ANALYSIS OF INFECTION AGENTS OF FARM ANIMALS ANAPLASMOSIS ON THE TERRITORY OF WESTERN AND EASTERN SIBERIA

V. A. Par,
T. I. Yepikhina,
E. A. Yefremova,
V. A. Marchenko,
O. V. Suntsova,
O. V. Lisak,
E. K. Doroshchenko,
I. M. Zubareva,
A. Y. Tikunov,
N. V. Tikunova

Affiliations

V. A. Par: Institute of Chemical Biology and Fundamental Medicine, SB RAS
T. I. Yepikhina: Institute of Chemical Biology and Fundamental Medicine, SB RAS
E. A. Yefremova: Institute of Experimental Veterinary Science of Siberia and Far East
V. A. Marchenko: Gorno-Altay Research Institute of Agriculture
O. V. Suntsova: Scientific Center for Family Health and Human Reproduction Problems
O. V. Lisak: Scientific Center for Family Health and Human Reproduction Problems
E. K. Doroshchenko: Scientific Center for Family Health and Human Reproduction Problems
I. M. Zubareva: Novosibirsk State Agrarian University
A. Y. Tikunov: Institute of Chemical Biology and Fundamental Medicine, SB RAS
N. V. Tikunova: Institute of Chemical Biology and Fundamental Medicine, SB RAS

Journal volume & issue: Vol. 0, no. 5
pp. 83 – 87

Abstract

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A total of 452 blood samples of cattle, sheep and goat collected in different regions of Altai Republic, Altai region, Novosibirsk and Irkutsk regions were examined on the presence of Anaplasma DNA using nested PCR with subsequent sequencing of PCR fragments. Anaplasma DNA was found in all examined blood samples of goat, 75.2 % samples of sheep, and 49.3 % samples of cattle. A molecular genetic analysis has demonstrated that intraerythrocytic Anaplasma ovis circulates in goat and sheep blood, while an intraerythrocytic Anaplasma sp. Omsk and a new intraerythrocytic Anaplasma sp. Sibl22, which cannot be attributed to any known species, circulate in cattle blood. In addition to intraerythrocytic Anaplasma, DNA of monocytic Anaplasma bovis was found in one blood sample of cattle.

Published in Acta Biomedica Scientifica

ISSN: 2541-9420 (Print); 2587-9596 (Online)
Publisher: Scientific Сentre for Family Health and Human Reproduction Problems
Country of publisher: Russian Federation
LCC subjects: Science
Website: http://actabiomedica.ru/en/

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