C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair

Abstract

Summary: Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples.For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021).

Keywords

• Cell biology
• Genetics
• Model organisms
• Molecular biology