Antifungal Activity of Cyclic Tetrapeptide from <i>Bacillus velezensis</i> CE 100 against Plant Pathogen <i>Colletotrichum gloeosporioides</i>
Vantha Choub,
Chaw Ei Htwe Maung,
Sang-Jae Won,
Jae-Hyun Moon,
Kil Yong Kim,
Yeon Soo Han,
Jeong-Yong Cho,
Young Sang Ahn
Affiliations
Vantha Choub
Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Chaw Ei Htwe Maung
Division of Agricultural and Biological Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Sang-Jae Won
Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Jae-Hyun Moon
Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Kil Yong Kim
Division of Agricultural and Biological Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Yeon Soo Han
Division of Plant Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Jeong-Yong Cho
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Young Sang Ahn
Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
The aim of this study was to investigate the antifungal activity of a cyclic tetrapeptide from Bacillus velezensis CE 100 against anthracnose-causing fungal pathogen Colletotrichum gloeosporioides. Antifungal compound produced by B. velezensis CE 100 was isolated and purified from ethyl acetate extract of B. velezensis CE 100 culture broth using octadecylsilane column chromatography. The purified compound was identified as cyclo-(prolyl-valyl-alanyl-isoleucyl) based on mass spectrometer and nuclear magnetic resonance analyses. This is the first report of the isolation of a cyclic tetrapeptide from B. velezensis CE 100 culture filtrate. Cyclic tetrapeptide displayed strong antifungal activity at concentration of 1000 µg/mL against C. gloeosporioides mycelial growth and spore germination. Our results demonstrate that the antifungal cyclic tetrapeptide from B. velezensis CE 100 has potential in bioprotection against anthracnose disease of plants caused by C. gloeosporioides.
