Antifungal and anti-mycotoxigenic activity of selected South African medicinal plants species

P.M. Dikhoba; N.I. Mongalo; E.E. Elgorashi; T.J. Makhafola

Heliyon (Oct 2019)

Antifungal and anti-mycotoxigenic activity of selected South African medicinal plants species

P.M. Dikhoba,
N.I. Mongalo,
E.E. Elgorashi,
T.J. Makhafola

Affiliations

P.M. Dikhoba: University of South Africa, College of Agriculture and Environmental Sciences (CAES), Department of Life and Consumer Sciences, Private Bag X06, Florida, Johannesburg, 0710, South Africa
N.I. Mongalo: University of South Africa, CAES Laboratories, Private Bag X06, Florida, Johannesburg, 1710, South Africa; Corresponding author.
E.E. Elgorashi: University of South Africa, College of Agriculture and Environmental Sciences (CAES), Department of Life and Consumer Sciences, Private Bag X06, Florida, Johannesburg, 0710, South Africa; ARC-Onderstepoort Veterinary Institute, Toxicology and Ethnoveterinary Medicine, Public Health and Zoonoses, Private Bag X05, Onderstepoort, 0110, South Africa
T.J. Makhafola: Central University of Technology, Faculty of Health and Environmental Sciences, Centre for Quality of Health and Living, Private Bag X20539, Bloemfontein, 20539, South Africa

Journal volume & issue: Vol. 5, no. 10
p. e02668

Abstract

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Antifungal and anti-mycotoxigenic activity of 25 acetone leaf extracts of South African medicinal plants with potent antioxidant activity were investigated against three phytopathogenic fungal strains. The extracts exhibited varying degrees of in vitro anti-mycotoxigenic effect against Fusarium verticillioides, Aspergillus flavus and Aspergillus ochraceous. Markhamia obtusifolia (Baker) Sprague exhibited the lowest minimum inhibitory concentration (MIC) values as low as 0.08 mg/ml against Aspergillus flavus and Furasium verticilloides at both 24 and 48 hr incubation period, while Curtisia dentata exhibited similar MIC value against Aspergillus ochraceous. Curtisia dentata further yielded the highest total activity of 1583 ml/g against Aspergillus ochraceous at 24 and 48 hr incubation period. In the mycelial growth inhibition (MGI) evaluation, Fusarium verticilloides was more sensitive to plants extracts, while Kirkia wilmsii exhibited highest MGI of 50.08% against Fusarium verticilloides on the 6th day of incubation. Five acetone extracts from Acokanthora oppositifolia, Bauhinia galpinii, Combretum caffrum, Ricinus communis and Solanum aculeastrum exhibited lowest IC50 value of 0.01 mg/ml against (2,2’-azinobis-3-ethylbenzthiazoline-6-suphonic acid (ABTS). Curtisia dentata and Markhamia obtusifolia extracts were further subjected to gas chromatography mass-spectrophotometry (GC-MS) analysis. Curtisia dentata revealed the presence of triterpenoid compounds, β-amyrin (53.30%) and α-amyrin (6.42%), while Markhamia obtusifolia yielded the presence of neophytadiene (4.38%) and palmitic acid (3.61%) The results suggest that natural products from plants may well be used as possible substitutes for synthetic fungicides. Given the antifungal and antioxidant potential of the selected plants, they may have potential as possible leads for the development of biofungicides that may well prevent oxidation related food spoilage.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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