Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9

Chun-Lin Liu; Ching-Ying Huang; Hung-Chih Chen; Huai-En Lu; Patrick C.H. Hsieh; Jia-Jung Lee

Stem Cell Research (May 2020)

Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9

Chun-Lin Liu,
Ching-Ying Huang,
Hung-Chih Chen,
Huai-En Lu,
Patrick C.H. Hsieh,
Jia-Jung Lee

Affiliations

Chun-Lin Liu: Human Disease iPSC Service Consortium, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Ching-Ying Huang: Human Disease iPSC Service Consortium, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Hung-Chih Chen: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Huai-En Lu: Food Industry Research and Development Institute, Hsinchu, Taiwan
Patrick C.H. Hsieh: Human Disease iPSC Service Consortium, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Jia-Jung Lee: Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Faculty of Renal Care, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author at: Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Medical University, Kaohsiung, Taiwan.

Journal volume & issue: Vol. 45

Abstract

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We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal