RELATIVE EXPRESSION OF GENES CHIA1, SGF14C AND CHS8* IN SOYBEAN SEED COATS

Abstract

The aim of this study was to evaluate the relative expression of three candidate genes, CHIA1, SGF14c and CHS8 * possibly involved in seed quality, in contrasting seed coats from four soybean genotypes. Two genotypes with yellow seed coats, BMX Potência RR and CD 202, and two genotypes with black seed coats, TP and IAC were studied to determine the relative gene expression through the qPCR technique, in sev- en stages of seed coat development for all four genotypes, at 25, 30, 35, 40, 45, 50 and 55 days after anthesis. The CHIA1 and SGF14c genes showed higher expression in cultivar CD 202; the former in the final stages of seed coat development, at 55 days after anthesis, the latter gene at earlier stages, specifically at 25 days after anthesis. The CHS8* gene showed higher expression in CD 202 seed coats at 50 days after anthesis. All three genes expressed at higher levels on genotypes of yellow seed coats, and are considered relevant to new areas of research based on the expression of genes related to seed quality.