In vitro morphogenesis ofPhysalis ixocarpaBrot ex. Horm.

Abstract

Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue cultureis pointed out as a strategy for its propagation, but researches indicate that invitroresponses are genotype-dependent.This study aimed to evaluate the in vitromorphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitromorphogeneticexpression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied.

Keywords

• solanaceae
• husk tomato
• plant propagation
• tissue culture