OncoTargets and Therapy (Dec 2019)
LncRNA FTX Promotes Proliferation and Invasion of Gastric Cancer via miR-144/ZFX Axis
Abstract
Hongbo Li,1 Guoliang Yao,2 Jingming Zhai,2 Dingwen Hu,2 Yonggang Fan2 1College of Clinical Medicine, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province 471003, People’s Republic of China; 2Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province 471000, People’s Republic of ChinaCorrespondence: Yonggang FanHepatobiliary Pancreatic Surgery, The First Affiliated Hospital of Henan University of Science and Technology, No. 636 Guanlin Road, Luolong District, Luoyang, Henan Province 471000, People’s Republic of ChinaTel +86 159 3799 0302Email [email protected]: Long non-coding RNAs are important regulators in cancer cell tumorigenesis. We have demonstrated in a prior study that lncRNA FTX is dysregulated in gastric cancer (GC). In this study, we aim to report gastric cancer-related lncRNA FTX as a main regulator in GC development and progression.Methods: In vitro and in vivo assays of FTX alterations have been performed to reveal a complex integrated phenotype affecting cell growth, migration, and invasion. lncRNA FTX expression levels in gastric cancer cells and normal cells were measured by RT-PCR. Luciferase reporter assays, Western blotting, and many immune, microscopy technologies were utilized to investigate the expressions of FTX- related proteins and RNAs. The functional role of FTX in cell growth, migration, and invasion were observed in vitro and in vivo.Results: We explored the underlying mechanisms of FTX in GC development, and the microRNAs’ relationship with FTX. We found that FTX promoted cell proliferation, migration, and invasion, as well as tumor growth, and this effect could latterly be attenuated by miR-144. ZFX attenuated the effects of FTX/miR-144 axis by sponging with miR-144.Conclusion: In summary, the above results support a model in which the FTX/miR-144/ZFX act as important effectors in GC tumorigenesis and progression, indicating new therapeutic methods in GC.Keywords: gastric cancer, LncRNA FTX, proliferation, invasion, miR-144/ZFX axis
