مجلة كلية الطب (Jan 2007)
DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method
Abstract
Background: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration. Design: Prospective observational study. Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted through years 2004-2005. Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (μg/ml) was estimated using a microchemical spectrophotometric method. Results: The three groups were statistically of significant difference( P 0.05 )correlation was observed in the other two groups (Gr. I & Gr. II ). Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content. in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients . D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients.
