Nature Communications (May 2021)
INI1/SMARCB1 Rpt1 domain mimics TAR RNA in binding to integrase to facilitate HIV-1 replication
- Updesh Dixit,
- Savita Bhutoria,
- Xuhong Wu,
- Liming Qiu,
- Menachem Spira,
- Sheeba Mathew,
- Richard Harris,
- Lucas J. Adams,
- Sean Cahill,
- Rajiv Pathak,
- P. Rajesh Kumar,
- Minh Nguyen,
- Seetharama A. Acharya,
- Michael Brenowitz,
- Steven C. Almo,
- Xiaoqin Zou,
- Alasdair C. Steven,
- David Cowburn,
- Mark Girvin,
- Ganjam V. Kalpana
Affiliations
- Updesh Dixit
- Department of Genetics, Albert Einstein College of Medicine
- Savita Bhutoria
- Department of Genetics, Albert Einstein College of Medicine
- Xuhong Wu
- Department of Genetics, Albert Einstein College of Medicine
- Liming Qiu
- Dalton Cardiovascular Research Center, Department of Physics and Astronomy, Department of Biochemistry, and Institute for Data Science and Informatics, University of Missouri
- Menachem Spira
- Department of Genetics, Albert Einstein College of Medicine
- Sheeba Mathew
- Department of Genetics, Albert Einstein College of Medicine
- Richard Harris
- Department of Biochemistry, Albert Einstein College of Medicine
- Lucas J. Adams
- Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
- Sean Cahill
- Department of Biochemistry, Albert Einstein College of Medicine
- Rajiv Pathak
- Department of Genetics, Albert Einstein College of Medicine
- P. Rajesh Kumar
- Department of Biochemistry, Albert Einstein College of Medicine
- Minh Nguyen
- Department of Genetics, Albert Einstein College of Medicine
- Seetharama A. Acharya
- Department of Anatomy & Structural Biology, Albert Einstein College of Medicine
- Michael Brenowitz
- Department of Biochemistry, Albert Einstein College of Medicine
- Steven C. Almo
- Department of Biochemistry, Albert Einstein College of Medicine
- Xiaoqin Zou
- Dalton Cardiovascular Research Center, Department of Physics and Astronomy, Department of Biochemistry, and Institute for Data Science and Informatics, University of Missouri
- Alasdair C. Steven
- Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
- David Cowburn
- Department of Biochemistry, Albert Einstein College of Medicine
- Mark Girvin
- Department of Biochemistry, Albert Einstein College of Medicine
- Ganjam V. Kalpana
- Department of Genetics, Albert Einstein College of Medicine
- DOI
- https://doi.org/10.1038/s41467-021-22733-9
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
HIV-1 integrase (IN) binds the host factor INI1/SMARCB1, which is required at multiple stages of HIV-1 replication. Here, the authors show that the same IN residues are involved in INI1 and RNA binding and in influencing particle morphogenesis and suggest that the IN-binding INI1 domain is structurally similar to HIV TAR RNA.
