Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome

Abstract

This research aimed to prove the difference of TLR4 and p65NF-kB subunit expression between children with steroid -sensitive nephrotic syndrome (SSNS) and those with steroid-resistent nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients’ blood samples, followed by monocyte isolation of PMBC using monocyte culture. Examination of TLR4 and p65 NF-kB expression from monocyte's cells culture was performed using flow cytometry. The result showed that the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), The differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65 NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p<0.05). There is a significant difference of TLR4 expression between SRNS and SSNS and there is also a significant correlation between expression of TLR4 and SRNS, that proved TLR4 has an important role in steroid resistance of nephrotic syndrome.

Keywords

• INS
• SSNS
• SRNS
• TLR4 expression
• p65 NF - kB expression