PLoS ONE (Jan 2014)

Single-step selection of bivalent aptamers validated by comparison with SELEX using high-throughput sequencing.

  • Robert Wilson,
  • Christian Bourne,
  • Roy R Chaudhuri,
  • Richard Gregory,
  • John Kenny,
  • Andrew Cossins

DOI
https://doi.org/10.1371/journal.pone.0100572
Journal volume & issue
Vol. 9, no. 6
p. e100572

Abstract

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The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potential to simplify the selection of aptamers by avoiding multiple rounds of enzymatic transcription and amplification.