Plasmon resonance biosensor for interleukin-1β point-of-care determination: A tool for early periodontitis diagnosis
Nunzio Cennamo,
Debora Bencivenga,
Marco Annunziata,
Francesco Arcadio,
Emanuela Stampone,
Angelantonio Piccirillo,
Fulvio Della Ragione,
Luigi Zeni,
Luigi Guida,
Adriana Borriello
Affiliations
Nunzio Cennamo
Department of Engineering, University of Campania “Luigi Vanvitelli”, Via Roma, 9, Aversa, CE 81031, Italy
Debora Bencivenga
Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, via De Crecchio, 7 80138 Naples, Italy
Marco Annunziata
Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania “Luigi Vanvitelli”, via De Crecchio, 6 80138 Naples, Italy
Francesco Arcadio
Department of Engineering, University of Campania “Luigi Vanvitelli”, Via Roma, 9, Aversa, CE 81031, Italy
Emanuela Stampone
Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, via De Crecchio, 7 80138 Naples, Italy
Angelantonio Piccirillo
Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania “Luigi Vanvitelli”, via De Crecchio, 6 80138 Naples, Italy
Fulvio Della Ragione
Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, via De Crecchio, 7 80138 Naples, Italy
Luigi Zeni
Department of Engineering, University of Campania “Luigi Vanvitelli”, Via Roma, 9, Aversa, CE 81031, Italy; Corresponding author
Luigi Guida
Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania “Luigi Vanvitelli”, via De Crecchio, 6 80138 Naples, Italy; Corresponding author
Adriana Borriello
Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, via De Crecchio, 7 80138 Naples, Italy; Corresponding author
Summary: Among pro-inflammatory cytokines, Interleukin-1β is crucially involved in several inflammatory-based diseases and even cancer. Increased Interleukin-1β levels in oral fluids have been proposed as an early marker of periodontitis, a broadly diffused chronic inflammatory condition of periodontal-supporting tissues, leading eventually to tooth loss. We describe the development of a portable surface-plasmon-resonance-based optical fiber probe suitably coated with an anti-Interleukin-1β antibody monolayer. A pico-nanomolar linear range of determination was obtained in both buffer solution and saliva with a rapid (3 min) incubation and high selectivity in presence of interferents. Higher Interleukin-1β concentration in the saliva of a periodontitis patient compared to a healthy control was determined. These measurements were validated by an automated ELISA system. Our results sustain the potential applicability of the proposed SPR-POF as diagnostic point-of-care device for real-time monitoring of salivary Interleukin-1β, that can support early detection of oral inflammatory-based pathologies and rapid and timely therapeutic decisions.
