Genetic and molecular studies on components of rust resistance in recombinant inbred lines and back-cross populations of peanut (Arachis hypogaea L.)

Abstract

Recombinant inbred lines and backcross populations of GPBD 5 × ICGV 86699 were inoculated with uredospores of rust fungus to study the genetic variation of components of rust resistance, relationships among components of resistance to rust and identification of microsatellite markers linked to rust resistance in peanut. There were highly significant differences among recombinants for incubation period and number of pustules per leaf area in all the population and high genetic gain along with high heritability for all components of rust resistance. Association study revealed all the components of rust resistance were significantly correlated with each other except Incubation period. Bulk segregant analysis in the segregating populations of cross (GPBD 5 × ICGV 86699) indicated SSR marker TC4g10 to be putatively linked to rust resistance. Further, validation of this marker outside the original mapping population could strengthen the reliable association of this marker with rust.

Keywords

• components of rust resistance
• puccinia arachidis
• backcross breeding
• recombinant inbred lines
• ssr marker