PLoS ONE (Jan 2012)

Discovery and genomic characterization of noroviruses from a gastroenteritis outbreak in domestic cats in the US.

  • Pierfrancesco Pinto,
  • Qiuhong Wang,
  • Ning Chen,
  • Edward J Dubovi,
  • Joshua B Daniels,
  • Laurie M Millward,
  • Canio Buonavoglia,
  • Vito Martella,
  • Linda J Saif

DOI
https://doi.org/10.1371/journal.pone.0032739
Journal volume & issue
Vol. 7, no. 2
p. e32739

Abstract

Read online

Norovirus (NoV) RNA was detected in the stools of 6 out 14 (42.8%) 8-12-week-old cats with enteritis from a feline shelter, in New York State. Upon sequence analysis of the complete capsid, the six NoVs were found to be identical, suggesting the spread of a unique NoV strain in the shelter. The full-length genomic sequence (7839 nt) of one feline NoV, CU081210E/2010/US, was determined. In the capsid protein VP1 region, the virus displayed the highest amino acid identity to animal genogroup IV genotype 2 (GIV.2) NoVs: lion/Pistoia-387/06/IT (97.9%) and dog/Bari-170/07/IT (90.4%). These findings document the discovery of a novel feline calicivirus, different from vesiviruses, and extend the spectrum of NoV host range. Epidemiological studies using feline NoV-specific diagnostic tools and experimental infection of cats are required to understand whether NoVs have a pathogenic role in this species.