The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks

Ann-Marie K. Shorrocks; Samuel E. Jones; Kaima Tsukada; Carl A. Morrow; Zoulikha Belblidia; Johanna Shen; Iolanda Vendrell; Roman Fischer; Benedikt M. Kessler; Andrew N. Blackford

doi:10.1038/s41467-020-20818-5

Nature Communications (Jan 2021)

The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks

Ann-Marie K. Shorrocks,
Samuel E. Jones,
Kaima Tsukada,
Carl A. Morrow,
Zoulikha Belblidia,
Johanna Shen,
Iolanda Vendrell,
Roman Fischer,
Benedikt M. Kessler,
Andrew N. Blackford

Affiliations

Ann-Marie K. Shorrocks: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Samuel E. Jones: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Kaima Tsukada: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Carl A. Morrow: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Zoulikha Belblidia: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Johanna Shen: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital
Iolanda Vendrell: MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford
Roman Fischer: Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford
Benedikt M. Kessler: Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford
Andrew N. Blackford: MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital

DOI: https://doi.org/10.1038/s41467-020-20818-5
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The BLM helicase interacts with the topoisomerase TOP3A and RMI1 to form the BTR complex. Here, the authors reveal that this complex contains multiple binding sites for the single-stranded DNA-binding complex RPA, and that RPA-binding stimulates BLM recruitment to stalled replication forks to promote their restart after replication stress.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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