Nature Communications (Jan 2021)

The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks

  • Ann-Marie K. Shorrocks,
  • Samuel E. Jones,
  • Kaima Tsukada,
  • Carl A. Morrow,
  • Zoulikha Belblidia,
  • Johanna Shen,
  • Iolanda Vendrell,
  • Roman Fischer,
  • Benedikt M. Kessler,
  • Andrew N. Blackford

DOI
https://doi.org/10.1038/s41467-020-20818-5
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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The BLM helicase interacts with the topoisomerase TOP3A and RMI1 to form the BTR complex. Here, the authors reveal that this complex contains multiple binding sites for the single-stranded DNA-binding complex RPA, and that RPA-binding stimulates BLM recruitment to stalled replication forks to promote their restart after replication stress.