npj Regenerative Medicine (Nov 2021)
Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes
- Frank A. Petrigliano,
- Nancy Q. Liu,
- Siyoung Lee,
- Jade Tassey,
- Arijita Sarkar,
- Yucheng Lin,
- Liangliang Li,
- Yifan Yu,
- Dawei Geng,
- Jiankang Zhang,
- Ruzanna Shkhyan,
- Jacob Bogdanov,
- Ben Van Handel,
- Gabriel B. Ferguson,
- Youngjoo Lee,
- Svenja Hinderer,
- Kuo-Chang Tseng,
- Aaron Kavanaugh,
- J. Gage Crump,
- April D. Pyle,
- Katja Schenke-Layland,
- Fabrizio Billi,
- Liming Wang,
- Jay Lieberman,
- Mark Hurtig,
- Denis Evseenko
Affiliations
- Frank A. Petrigliano
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Nancy Q. Liu
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Siyoung Lee
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Jade Tassey
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Arijita Sarkar
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Yucheng Lin
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Liangliang Li
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Yifan Yu
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Dawei Geng
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Jiankang Zhang
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Ruzanna Shkhyan
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Jacob Bogdanov
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Ben Van Handel
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Gabriel B. Ferguson
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Youngjoo Lee
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Svenja Hinderer
- The Natural and Medical Sciences Institute (NMI) at the University of Tübingen
- Kuo-Chang Tseng
- Department of Stem Cell Research and Regenerative Medicine, USC
- Aaron Kavanaugh
- Department of Orthopaedic Surgery, David Geffen School of Medicine, UCLA
- J. Gage Crump
- Department of Stem Cell Research and Regenerative Medicine, USC
- April D. Pyle
- Department of Microbiology, Immunology, and Molecular Genetics, UCLA
- Katja Schenke-Layland
- The Natural and Medical Sciences Institute (NMI) at the University of Tübingen
- Fabrizio Billi
- Department of Orthopaedic Surgery, David Geffen School of Medicine, UCLA
- Liming Wang
- Department of Orthopaedic Surgery, Nanjing First Hospital, Nanjing Medical University
- Jay Lieberman
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- Mark Hurtig
- Ontario Veterinary College, Department of Clinical Studies, University of Guelph
- Denis Evseenko
- Department of Orthopaedic Surgery, Keck School of Medicine of USC, University of Southern California (USC)
- DOI
- https://doi.org/10.1038/s41536-021-00187-3
- Journal volume & issue
-
Vol. 6,
no. 1
pp. 1 – 14
Abstract
Abstract Osteoarthritis (OA) impacts hundreds of millions of people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for the development of OA. Current cartilage repair strategies are moderately effective at reducing pain but often replace damaged tissue with biomechanically inferior fibrocartilage. Here we describe the development, transcriptomic ontogenetic characterization and quality assessment at the single cell level, as well as the scaled manufacturing of an allogeneic human pluripotent stem cell-derived articular chondrocyte formulation that exhibits long-term functional repair of porcine articular cartilage. These results define a new potential clinical paradigm for articular cartilage repair and mitigation of the associated risk of OA.
