Studying the Effect of Gold Nanoparticles Prepared by Pulse Laser Ablation in a Liquid on Bacterial Activity

Abstract

Gold nanoparticles (AuNPs) were synthesized using the pulsed laser ablation technique in liquid. An (Nd: YAG) laser operating at a fixed energy of 600 mJ and a frequency of 4.3 Hz was employed. The absorption spectrum of the synthesized particles was measured using an ultraviolet-visible spectrometer (uv-vis), with peak values observed in the range of (523–530) nm. Scanning electron microscope (SEM) analysis results revealed that the particles exhibited a spherical shape and had an average size ranging from (38 - 92) nm. X-ray diffraction confirmed the crystalline structure of the material, which was determined to be cubic. Transmission electron microscopy (TEM) images demonstrated that the particle diameter was (31 and 21) nm for the respective frequencies, which corresponded to 1200 pulses. Furthermore, the biological activity of the AuNPs was assessed against positive and negative bacteria.

Keywords

• pulsed laser ablation technique, AuNPs, (UV-Vis) spectroscopy, SEM, TEM