Медицинская иммунология (Aug 2019)

EFFECT OF RIFAMPICIN ON TOLL-LIKE RECEPTOR SYSTEM GENE EXPRESSION IN BRAIN STRUCTURES OF RATS WITH PRENATAL ALCOHOL EXPOSURE

  • Marat Igorevich Airapetov,
  • Sergey Olegovich Eresko,
  • Alexandra Antonovna Mikhailova,
  • Daria Dmitrievna Sukhanova,
  • Polina Denisovna Ignatova,
  • Andrey Andreevich Lebedev,
  • Evgeny Rudolfovich Bychkov,
  • Petr Dmitrievich Shabanov

DOI
https://doi.org/10.15789/1563-0625-EOR-2935
Journal volume & issue
Vol. 0, no. 0

Abstract

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Alcohol intake during pregnancy may affect the normal course of fetal development, leading to the formation of symptoms of fetal alcohol spectrum disorders (FASD). There is evidence that a number of prenatal pathologic conditions exhibit alterations in the regulation of expression mechanisms of several pro- and anti-inflammatory cytokines. In our study, we focused on examining the expression levels of a number of pro- and anti-inflammatory cytokine genes in the forebrain and temporal regions of rat brain during the postnatal developmental period when modeling prenatal alcohol exposure (PAE). We also evaluated the expression level of genes that are associated with the regulation of gene expression of pro- and anti-inflammatory cytokines. In addition, the objectives of our study included pharmacological correction of the observed changes by a potential pharmacological agent - rifampicin, which according to other studies had a neuroprotective effect. The PAE was modeled by mating pregnant female rats with a 15% ethanol solution throughout pregnancy. Injections were performed in rats from the 1st to the 7th postnatal days. Brain structures were sampled for gene expression analysis on the 8th postnatal day. The results of the study showed the presence of changes in the expression of Tlr3 and Tlr4 genes on the 8th day of postnatal development in the anterior and temporal lobes of the brain. The expression of Myd88 and Ticam genes had multidirectional changes among the studied brain structures of PAE rats. The increased mRNA level of proinflammatory genes was noted. The use of Rif in experiments showed the ability of Rif (50 mg/kg) to correct the observed long-term pathological changes in the expression of the genes under study. It is of interest to study the dose-dependent effect in the future, as well as to investigate the revealed changes by protein methods. In addition, it seems important in the future to study the TLR signaling system in other brain structures with PAE, as well as at different terms of postnatal development in ontogenesis.

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