Effects of salinity and alkalinity on fatty acids, free amino acids and related substance anabolic metabolism of Nile tilapia

Yamei Cheng; Jinliang Zhao; Christian Larbi Ayisi; Xiaoying Cao

Aquaculture and Fisheries (Jul 2022)

Effects of salinity and alkalinity on fatty acids, free amino acids and related substance anabolic metabolism of Nile tilapia

Yamei Cheng,
Jinliang Zhao,
Christian Larbi Ayisi,
Xiaoying Cao

Affiliations

Yamei Cheng: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China; Center for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China
Jinliang Zhao: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China; Center for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural, Shanghai Ocean University, Shanghai, 201306, China.
Christian Larbi Ayisi: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China; Center for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Department of Fisheries and Aquatic Resources Management, Faculty of Natural Resources and Environment, University for Development Studies, Tamale, Ghana
Xiaoying Cao: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China; Center for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China

Journal volume & issue: Vol. 7, no. 4
pp. 389 – 395

Abstract

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A 56-day experiment was conducted to investigate the effects of ambient salinity and alkalinity on fatty acids, free amino acids and related anabolic metabolisms of Nile tilapia. The fatty acid and free amino acid contents in the muscle of tilapia cultured in saline water (SW), alkaline water (AW), saline–alkaline water (SAW) and fresh water (FW) conditions were analyzed and compared. The mRNA levels and enzymatic activities of fatty acyl desaturase (FADS2), elongation of very long-chain fatty acids (ELOVL5), Δ1-pyrroline-5-carboxylate synthase (P5CS), ornithine-δ-aminotransferase (δ-OAT), and proline dehydrogenase (PDH) were also determined. The contents of saturated and monounsaturated fatty acids were significantly higher in the AW and SAW groups than those in the SW group, and those of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were the highest in the SAW group. The contents of flavor amino acids in the SAW and AW groups were significantly higher than that in the SW group, and that of proline in the SW, AW, and SAW groups was 3.58-, 4.11-, and 4.15-times higher than that in the FW group. The mRNA levels and enzyme activities of FADS 2, ELOVL5, PDH and P5CS were found to differ among the different groups. The results suggested that ambient salinity and alkalinity could effectively improve flesh quality of tilapia by regulating lipid and proline metabolism pathways.

Published in Aquaculture and Fisheries

ISSN: 2468-550X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.keaipublishing.com/en/journals/aquaculture-and-fisheries

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