Ribonucleotide incorporation in yeast genomic DNA shows preference for cytosine and guanosine preceded by deoxyadenosine

Sathya Balachander; Alli L. Gombolay; Taehwan Yang; Penghao Xu; Gary Newnam; Havva Keskin; Waleed M. M. El-Sayed; Anton V. Bryksin; Sijia Tao; Nicole E. Bowen; Raymond F. Schinazi; Baek Kim; Kyung Duk Koh; Fredrik O. Vannberg; Francesca Storici

doi:10.1038/s41467-020-16152-5

Nature Communications (May 2020)

Ribonucleotide incorporation in yeast genomic DNA shows preference for cytosine and guanosine preceded by deoxyadenosine

Sathya Balachander,
Alli L. Gombolay,
Taehwan Yang,
Penghao Xu,
Gary Newnam,
Havva Keskin,
Waleed M. M. El-Sayed,
Anton V. Bryksin,
Sijia Tao,
Nicole E. Bowen,
Raymond F. Schinazi,
Baek Kim,
Kyung Duk Koh,
Fredrik O. Vannberg,
Francesca Storici

Affiliations

Sathya Balachander: School of Biological Sciences, Georgia Institute of Technology
Alli L. Gombolay: School of Biological Sciences, Georgia Institute of Technology
Taehwan Yang: School of Biological Sciences, Georgia Institute of Technology
Penghao Xu: School of Biological Sciences, Georgia Institute of Technology
Gary Newnam: School of Biological Sciences, Georgia Institute of Technology
Havva Keskin: School of Biological Sciences, Georgia Institute of Technology
Waleed M. M. El-Sayed: School of Biological Sciences, Georgia Institute of Technology
Anton V. Bryksin: Molecular Evolution Core, Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology
Sijia Tao: Department of Pediatrics, School of Medicine, Emory University
Nicole E. Bowen: Department of Pediatrics, School of Medicine, Emory University
Raymond F. Schinazi: Department of Pediatrics, School of Medicine, Emory University
Baek Kim: Department of Pediatrics, School of Medicine, Emory University
Kyung Duk Koh: Lung Biology Center, Department of Medicine, University of California San Francisco
Fredrik O. Vannberg: School of Biological Sciences, Georgia Institute of Technology
Francesca Storici: School of Biological Sciences, Georgia Institute of Technology

DOI: https://doi.org/10.1038/s41467-020-16152-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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Ribonucleoside monophosphates are incorporated by DNA polymerases into double-stranded DNA. Here, the authors use ribose-seq and Ribose-Map techniques to reveal that signatures and patterns of ribonucleotide incorporation in yeast mitochondrial and nuclear DNA show preference for cytosine and guanosine preceded by deoxyadenosine.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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