Keep it accurate and robust: An enhanced nuclei analysis framework

Abstract

Accurate segmentation and classification of nuclei in histology images is critical but challenging due to nuclei heterogeneity, staining variations, and tissue complexity. Existing methods often struggle with limited dataset variability, with patches extracted from similar whole slide images (WSI), making models prone to falling into local optima. Here we propose a new framework to address this limitation and enable robust nuclear analysis. Our method leverages dual-level ensemble modeling to overcome issues stemming from limited dataset variation. Intra-ensembling applies diverse transformations to individual samples, while inter-ensembling combines networks of different scales. We also introduce enhancements to the HoVer-Net architecture, including updated encoders, nested dense decoding and model regularization strategy. We achieve state-of-the-art results on public benchmarks, including 1st place for nuclear composition prediction and 3rd place for segmentation/classification in the 2022 Colon Nuclei Identification and Counting (CoNIC) Challenge. This success validates our approach for accurate histological nuclei analysis. Extensive experiments and ablation studies provide insights into optimal network design choices and training techniques. In conclusion, this work proposes an improved framework advancing the state-of-the-art in nuclei analysis. We will release our code and models to serve as a toolkit for the community.

Keywords

• Digital pathology
• Deep learning
• Nuclei segmentation
• Nuclei classification