Nature Communications (Mar 2017)
Global site-specific N-glycosylation analysis of HIV envelope glycoprotein
- Liwei Cao,
- Jolene K. Diedrich,
- Daniel W. Kulp,
- Matthias Pauthner,
- Lin He,
- Sung-Kyu Robin Park,
- Devin Sok,
- Ching Yao Su,
- Claire M. Delahunty,
- Sergey Menis,
- Raiees Andrabi,
- Javier Guenaga,
- Erik Georgeson,
- Michael Kubitz,
- Yumiko Adachi,
- Dennis R. Burton,
- William R. Schief,
- John R. Yates III,
- James C. Paulson
Affiliations
- Liwei Cao
- Department of Cell and Molecular Biology, The Scripps Research Institute
- Jolene K. Diedrich
- Department of Chemical Physiology, The Scripps Research Institute
- Daniel W. Kulp
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Matthias Pauthner
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Lin He
- Department of Chemical Physiology, The Scripps Research Institute
- Sung-Kyu Robin Park
- Department of Chemical Physiology, The Scripps Research Institute
- Devin Sok
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Ching Yao Su
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Claire M. Delahunty
- Department of Chemical Physiology, The Scripps Research Institute
- Sergey Menis
- Department of the IAVI Neutralizing Antibody Center, The Scripps Research Institute
- Raiees Andrabi
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Javier Guenaga
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Erik Georgeson
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Michael Kubitz
- Department of Immunology and Microbial Science, The Scripps Research Institute
- Yumiko Adachi
- Department of the IAVI Neutralizing Antibody Center, The Scripps Research Institute
- Dennis R. Burton
- Department of Immunology and Microbial Science, The Scripps Research Institute
- William R. Schief
- Department of Immunology and Microbial Science, The Scripps Research Institute
- John R. Yates III
- Department of Chemical Physiology, The Scripps Research Institute
- James C. Paulson
- Department of Cell and Molecular Biology, The Scripps Research Institute
- DOI
- https://doi.org/10.1038/ncomms14954
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 13
Abstract
The analysis of site-specific glycosylation of HIV Envelope glycoprotein (Env) is challenging as it contains 25–30 glycosylation sites with multiple glycan forms at each site. Here the authors present a generally applicable mass spectrometry-based method for site-specific analysis of protein glycosylation that they apply to the analysis of the HIV-1 Env.
