Comparative Transcriptome Analysis Identifies MAPK Signaling Pathway Associated with Regulating Ovarian Lipid Metabolism during Vitellogenesis in the Mud Crab, <i>Scylla paramamosain</i>
Yuanhao Ren,
Wei Wang,
Yin Fu,
Zhiqiang Liu,
Ming Zhao,
Likun Xu,
Tianyong Zhan,
Ting Huang,
Minghao Luo,
Wei Chen,
Chunyan Ma,
Fengying Zhang,
Keji Jiang,
Lingbo Ma
Affiliations
Yuanhao Ren
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Wei Wang
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Yin Fu
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Zhiqiang Liu
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Ming Zhao
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Likun Xu
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Tianyong Zhan
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Ting Huang
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Minghao Luo
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Wei Chen
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Chunyan Ma
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Fengying Zhang
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Keji Jiang
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
Lingbo Ma
Key Laboratory of East China Fishery Resources Exploitation, East China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Science, Shanghai 200090, China
The mud crab, Scylla paramamosain, has abundant nutrients in the ovary, where numerous lipids accumulate during ovarian maturation. However, the mechanism behind the accumulation of lipids in the ovary of mud crab during ovarian maturation is largely unknown. This study conducted a comparative transcriptome analysis of the ovaries of mud crabs at various stages of ovarian maturation. A total of 63.69 Gb of clean data was obtained, with a Q30 of 93.34%, and 81,893 unigenes were identified, including 10,996 differentially expressed genes (DEGs). After KEGG enrichment of these DEGs, MAPK signaling pathway was significantly enriched during vitellogenesis. Moreover, the expression levels of genes involved in carbohydrate, amino acid, and lipid metabolism were found to be higher during vitellogenesis. The two genes (Sp-Eip75B and Sp-Eip78C) that are homologous to the vertebrate gene PPARγ in the PPAR signaling pathway, were identified. Additionally, genes in MAPK signaling pathway might regulate lipid metabolism through PPAR signaling pathway based on Protein-Protein Interaction (PPI) network. These findings suggest that MAPK signaling pathway plays a critical role in lipid metabolism in the ovary during vitellogenesis, which provides new insights into the mechanism of lipid accumulation during ovarian maturation in mud crabs.
