A Rapid Screening Analysis of Antioxidant Compounds in Native Australian Food Plants Using Multiplexed Detection with Active Flow Technology Columns
Emmanuel Janaka Rochana Rupesinghe,
Andrew Jones,
Ross Andrew Shalliker,
Sercan Pravadali-Cekic
Affiliations
Emmanuel Janaka Rochana Rupesinghe
Food Science and Technology Research Group, School of Science and Health, Western Sydney University (Hawkesbury), Hawkesbury 1797, Australia
Andrew Jones
Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia
Ross Andrew Shalliker
Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia
Sercan Pravadali-Cekic
Australian Centre for Research on Separation Sciences (ACROSS), School of Science and Health, Western Sydney University (Parramatta), Room LZ.G.73, Corner of Pemberton Street and Victoria Road, Parramatta 2150, Australia
Conventional techniques for identifying antioxidant and phenolic compounds in native Australian food plants are laborious and time-consuming. Here, we present a multiplexed detection technique that reduces analysis time without compromising separation performance. This technique is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. Extracts from cinnamon myrtle (Backhousia myrtifolia) and lemon myrtle (Backhousia citriodora) leaves were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, mass spectroscopy (MS), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) derivatisation for antioxidants as detection methods. A number of antioxidant compounds were detected in the extracts of each leaf extract.
