INFLUENCE OF ANTHELMINTIC DRUGS ON ACTIVITIES OF 5'-NUCLEOTIDASE Bothriocephalus scorpii (Cestoda: Bothriocephalidae)

Abstract

Activities and properties of 5'-nucleotidase in the subcellular fractions of Bothriocephalus scorpii are studied with the use of adenosine monophosphate, inosine monophosphate and cytidine monophosphate as substrates. The high activity of 5'-nucleotidase was observed in the mitochondrial fraction. Dependence of the 5'-nucleotidase activity on the concentration of substrates and Mn2+ ions is investigated. Impact of various effectors and ions (sodium fluoride, EDTA, cysteine, K+, Na+ , Zn2+, Ca2+, Ba2+, Cu2+, Mn2+) on enzyme activity is determined. Effect of 10 anthelmintic drugs on enzyme activity has been tested. The most effective drugs are bitionol and oxinide.

Keywords

• 5'-nucleotidase
• adenosine monophosphate
• inosine monophosphate
• cytidine monophosphate
• mitochondria
• cestoda
• anthelmintic drugs