The impact of COL1A1 and COL6A1 expression on hypospadias and penile curvature severity
Prahara Yuri,
Gunadi,
Rahmadani Puji Lestari,
Firly Putri Fardilla,
Wiwit Ananda Wahyu Setyaningsih,
Nur Arfian,
Ishandono Dachlan
Affiliations
Prahara Yuri
Division of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital
Gunadi
Pediatric Surgery Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital
Rahmadani Puji Lestari
Division of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital
Firly Putri Fardilla
Division of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital
Wiwit Ananda Wahyu Setyaningsih
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
Nur Arfian
Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
Ishandono Dachlan
Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine,Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital
Abstract Background Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of darto tissue has been suggested as the pathophysiology of chordees. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expression of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature. Methods We included 60 children 60 degrees). Results qPCR showed that COL1A1 and COL6A1 expression was significantly downregulated in the distal (0.88 (0.38–2.53) and 0.54 (0.16–4.35), respectively) and proximal 0.76 (0.33–2.57) and 0.57 (0.18–1.38), respectively) hypospadias groups compared to controls (1.85 (0.24–4.61) and 0.93 (0.17–4.06), respectively) with p-values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expression (r = 0.458, p < 0.0001). Interestingly, COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe chordee groups compared to the mild chordee groups, with p-values of 0.003 and 0.037, respectively. Conclusions Aberrant COL1A1 and COL6A1 expression might affect abnormalities in darto tissue and penile curvature severity in hypospadias patients.
