MODULATION OF FUNCTIONAL MACROPHAGE ACTIVITY BY HLDF6 PEPTIDE UPON ADDING OPIATE RECEPTOR AGONISTS

Abstract

Abstract.The effect of a hexamer fragment of the peptide differentiation factor of the HL–60 cell line, HLDF6, on functional activity of C57Bl/6 mouse macrophages upon adding selective agonists of μ–, δ– and κ–type opiate receptors (DAGO, DADLE and Dynorphin (1–13) has been studied in the cell culture. It has been shown that opiate agonists were capable of enhancing adhesive properties and functional activity of macrophages during their direct interaction with cells. The intensity of stimulation depended on the type of the agonist and, respectively, the type of the opiate receptor. HLDF6 peptide enhanced macrophage activity after addition to the culture medium both at the initial cultivation stage and at the moment of phagosytosis induction. The data concerning the combined administration of HLDF6 and opiate agonists suggest the interaction between the peptide and the endogenous opiate system. (Med. Immunol., 2005, vol.7, № 1, pp 77584)

Keywords

• hldf6 peptide
• opiate agonists
• macrophages