Study on Transformation of The Gene of Resveratrol Synthase into Cordyceps militaris

Abstract

Resveratrol synthase (RS) is a key enzyme in Resveratrol (Res) synthesis pathway. RS genes have been transformed and expressed in many plants and microorganisms, and play biological roles in plant metabolism and regulation. In this study, the full length sequence of resveratrol synthase RS gene was transformed into Cordyceps militaris by Agrobacterium tumefaciens infestation with 1179 bp. The RS gene was integrated into Cordyceps militaris genome by PCR, Southern blot hybridization, Northern hybridization and other molecular biological tests. Objective To improve the content of RS gene in into Cordyceps militaris by transgenic technology, improve the resistance of Cordyceps militaris and promote the content of resveratrol downstream products, provide a theoretical basis for studying the biological function and regulation mechanism of RS gene metabolites, and provide an effective way to improve species, resistance and increase the yield of metabolites.