OsVIL2 Regulates Spikelet Development by Controlling Regulatory Genes in Oryza sativa

Hyeryung Yoon; Jungil Yang; Wanqi Liang; Dabing Zhang; Gynheung An

doi:10.3389/fpls.2018.00102

Frontiers in Plant Science (Feb 2018)

OsVIL2 Regulates Spikelet Development by Controlling Regulatory Genes in Oryza sativa

Hyeryung Yoon,
Jungil Yang,
Wanqi Liang,
Dabing Zhang,
Gynheung An

Affiliations

Hyeryung Yoon: Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, South Korea
Jungil Yang: Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, South Korea
Wanqi Liang: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Dabing Zhang: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Gynheung An: Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, South Korea

DOI: https://doi.org/10.3389/fpls.2018.00102
Journal volume & issue: Vol. 9

Abstract

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Flower organ patterning is accomplished by spatial and temporal functioning of various regulatory genes. We previously reported that Oryza sativa VIN3-LIKE 2 (OsVIL2) induces flowering by mediating the trimethylation of Histone H3 on LFL1 chromatin. In this study, we report that OsVIL2 also plays crucial roles during spikelet development. Two independent lines of T-DNA insertional mutants in the gene displayed altered organ numbers and abnormal morphology in all spikelet organs. Scanning electron microscopy showed that osvil2 affected organ primordia formation during early spikelet development. Expression analysis revealed that OsVIL2 is expressed in all stages of the spikelet developmental. Transcriptome analysis of developing spikelets revealed that several regulatory genes involved in that process and the formation of floral organs were down-regulated in osvil2. These results suggest that OsVIL2 is required for proper expression of the regulatory genes that control floral organ number and morphology.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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