Frontiers in Plant Science (Nov 2023)
Functional analysis of ZmG6PE reveals its role in responses to low-phosphorus stress and regulation of grain yield in maize
- Hongkai Zhang,
- Hongkai Zhang,
- Hongkai Zhang,
- Bowen Luo,
- Bowen Luo,
- Bowen Luo,
- Jin Liu,
- Jin Liu,
- Jin Liu,
- Xinwu Jin,
- Xinwu Jin,
- Xinwu Jin,
- Haiying Zhang,
- Haiying Zhang,
- Haiying Zhang,
- Haixu Zhong,
- Haixu Zhong,
- Haixu Zhong,
- Binyang Li,
- Binyang Li,
- Binyang Li,
- Hongmei Hu,
- Hongmei Hu,
- Hongmei Hu,
- Yikai Wang,
- Yikai Wang,
- Yikai Wang,
- Asif Ali,
- Asad Riaz,
- Javed Hussain Sahito,
- Javed Hussain Sahito,
- Muhammad Zafar Iqbal,
- Xiao Zhang,
- Xiao Zhang,
- Xiao Zhang,
- Dan Liu,
- Dan Liu,
- Dan Liu,
- Ling Wu,
- Ling Wu,
- Ling Wu,
- Duojiang Gao,
- Shiqiang Gao,
- Shunzong Su,
- Shibin Gao,
- Shibin Gao,
- Shibin Gao
Affiliations
- Hongkai Zhang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Hongkai Zhang
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Hongkai Zhang
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Bowen Luo
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Bowen Luo
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Bowen Luo
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Jin Liu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jin Liu
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Jin Liu
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Xinwu Jin
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xinwu Jin
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xinwu Jin
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Haiying Zhang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Haiying Zhang
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Haiying Zhang
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Haixu Zhong
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Haixu Zhong
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Haixu Zhong
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Binyang Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Binyang Li
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Binyang Li
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Hongmei Hu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Hongmei Hu
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Hongmei Hu
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Yikai Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yikai Wang
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Yikai Wang
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Asif Ali
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Asad Riaz
- Centre of Excellence for Plant Success in Nature and Agriculture, The Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, St. Lucia, Brisbane, QLD, Australia
- Javed Hussain Sahito
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Javed Hussain Sahito
- Key Laboratory of Wheat and Maize Crops Science, College of Agronomy, Henan Agricultural University, Zhengzhou, China
- Muhammad Zafar Iqbal
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xiao Zhang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xiao Zhang
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Xiao Zhang
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Dan Liu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Dan Liu
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Dan Liu
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Ling Wu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Ling Wu
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Ling Wu
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Duojiang Gao
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Shiqiang Gao
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Shunzong Su
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- Shibin Gao
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China
- Shibin Gao
- Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China
- Shibin Gao
- Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, Sichuan, China
- DOI
- https://doi.org/10.3389/fpls.2023.1286699
- Journal volume & issue
-
Vol. 14
Abstract
A previous metabolomic and genome-wide association analysis of maize screened a glucose-6-phosphate 1-epimerase (ZmG6PE) gene, which responds to low-phosphorus (LP) stress and regulates yield in maize’s recombinant inbred lines (RILs). However, the relationship of ZmG6PE with phosphorus and yield remained elusive. This study aimed to elucidate the underlying response mechanism of the ZmG6PE gene to LP stress and its consequential impact on maize yield. The analysis indicated that ZmG6PE required the Aldose_epim conserved domain to maintain enzyme activity and localized in the nucleus and cell membrane. The zmg6pe mutants showed decreased biomass and sugar contents but had increased starch content in leaves under LP stress conditions. Combined transcriptome and metabolome analysis showed that LP stress activated plant immune regulation in response to the LP stress through carbon metabolism, amino acid metabolism, and fatty acid metabolism. Notably, LP stress significantly reduced the synthesis of glucose-1-phosphate, mannose-6-phosphate, and β-alanine-related metabolites and changed the expression of related genes. ZmG6PE regulates LP stress by mediating the expression of ZmSPX6 and ZmPHT1.13. Overall, this study revealed that ZmG6PE affected the number of grains per ear, ear thickness, and ear weight under LP stress, indicating that ZmG6PE participates in the phosphate signaling pathway and affects maize yield-related traits through balancing carbohydrates homeostasis.
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