Challenges in the identification of Ochrobactrum anthropi in blood and sputum cultures of patients with cystic fibrosis

Abstract

Background and objectives: The Ochrobactrum anthropic identification is a challenge. In this context, we perform numerous methods to identify the O. anthropic and associated the achieved results among the methods. Methods: We performed the follow methods: manual phenotypic, Vitek®2, Phoenix™, polymerase chain reaction, MALDI-TOF Vitek MS™ and BioTyper™. Results: Biochemical tests showed limitations to identify O. anthropic. Vitek®2 was the only method that showed the same result seen in polymerase chain reaction for O. anthropic, in all samples, followed by Phoenix™. MALDI-MS™ failed at defining at species level, and MALDI-BD™ presented high diversity in identification, showing to be a test of low specificity. Conclusions: Each method showed advantages and disadvantages to identify the O. anthropic. Keywords: MALDI-TOF; PCR; Phoenix™; Ochrobactrum anthropic; Vitek®2