Nature Communications (Oct 2020)

A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics

  • Pan Fang,
  • Yanlong Ji,
  • Ivan Silbern,
  • Carmen Doebele,
  • Momchil Ninov,
  • Christof Lenz,
  • Thomas Oellerich,
  • Kuan-Ting Pan,
  • Henning Urlaub

DOI
https://doi.org/10.1038/s41467-020-19052-w
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification and a dedicated data processing tool.