مجله دانشکده دندانپزشکی اصفهان (Jan 2007)
Immunohistochemical Evaluation of P53 Expression in Dental Follicle and Dentigerous Cyst
Abstract
Introduction: The survey was aimed to immunohistochemical evaluation of P53 expression in dental follicle and dentigerous cyst according to the capability of creating neoplastic changes in dentigerous cyst.Methods and Materials: Twelve cases of dentigerous cyst which has been supplied from archives of oral and maxillofacial pathology department of Babol School of Dentistry, from March 2004 to March 2006 and twelve cases of dental follicle supplied from surgery of impacted third molar of people visited oral & maxillofacial surgery department of Babol School of Dentistry during 2006 were selected. Five microns slices were gotten from paraffin block stained with P53 antibody, using immunohistochemical procedure. The observing of all the stained slides was done according to H-score gradation; it has been considered a degree of staining intensity of epithelial cells and the percent of stained epithelial cells. Then the aggregate of these two elements announced as final score. The results have been analyzed with Mann Whitney test using SPSS soft ware.Results: The expression of P53 in dentigerous cyst was more than dental follicle. (p value = 0.001). In relation with the percent of cells stained by P53 (p value > 0.0001), this difference has been more than intensity of staining. (p value = 0.021).Conclusion: The expression of P53 in dentigerous cyst was more than dental follicle (p value < 0.05). It seems we can distribute this difference to the capability of creating of neoplastic changes in dentigerous cyst.Key words: P53, Dentigerous cyst, Dental follicle
