The physicochemical properties and molecular docking study of plasticized amphotericin B loaded sodium alginate, carboxymethyl cellulose, and gelatin-based films
Saurabh Bhatia,
Ahmed Al-Harrasi,
Ibrahim Hamza Almohana,
Mustafa Safa Albayati,
Muhammad Jawad,
Yasir Abbas Shah,
Sana Ullah,
Anil K. Philip,
Sobia Ahsan Halim,
Ajmal Khan,
Md Khalid Anwer,
Esra Koca,
Levent Yurdaer Aydemir,
Sevgin Dıblan
Affiliations
Saurabh Bhatia
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman; School of Health Science, University of Petroleum and Energy Studies, Dehradun, 248007, India; Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600077, India; Corresponding author. Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman.
Ahmed Al-Harrasi
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman; Corresponding author.
Ibrahim Hamza Almohana
School of Pharmacy, College of Health Sciences, University of Nizwa-616, Birkat Al Mouz, Oman
Mustafa Safa Albayati
School of Pharmacy, College of Health Sciences, University of Nizwa-616, Birkat Al Mouz, Oman
Muhammad Jawad
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman
Yasir Abbas Shah
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman
Sana Ullah
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman
Anil K. Philip
School of Pharmacy, College of Health Sciences, University of Nizwa-616, Birkat Al Mouz, Oman
Sobia Ahsan Halim
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman; Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa 616, Oman.
Ajmal Khan
Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa, 616, Oman
Md Khalid Anwer
Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, 11942, Saudi Arabia
Esra Koca
Department of Food Engineering, Adana Alparslan Turkes Science and Technology University, 01250, Adana, Turkey
Levent Yurdaer Aydemir
Department of Food Engineering, Adana Alparslan Turkes Science and Technology University, 01250, Adana, Turkey
Sevgin Dıblan
Food Processing Department, Vocational School of Technical Sciences at Mersin Tarsus Organized Industrial Zone, Tarsus University, 33100, Tarsus/Mersin, Turkey
Plasticizers are employed to stabilize films by safeguarding their physical stability and avoiding the degradation of the loaded therapeutic drug during processing and storage. In the present study, the plasticizer effect (glycerol) was studied on bioadhesive films based on sodium alginate (SA), carboxymethyl cellulose (CMC) and gelatin (GE) polymers loaded with amphotericin B (AmB). The main objective of the current study was to assess the morphological, mechanical, thermal, optical, and barrier properties of the films as a function of glycerol (Gly) concentration (0.5–1.5 %) using different techniques such as Scanning Electron Microscope (SEM), Texture analyzer (TA), Differential Scanning Calorimeter (DSC), X-Ray Diffraction (XRD), and Fourier Transforms Infrared Spectroscopy (FTIR). The concentration increase of glycerol resulted in an increase in Water Vapor Permeability (WVP) (0.187–0.334), elongation at break (EAB) (0.88–35.48 %), thickness (0.032–0.065 mm) and moisture level (17.5–41.76 %) whereas opacity, tensile strength (TS) (16.81–0.86 MPa), and young's modulus (YM) (0.194–0.002 MPa) values decreased. Glycerol incorporation in the film-Forming solution decreased the brittleness and fragility of the films. Fourier Transform Infrared (FTIR) spectra showed that intermolecular hydrogen bonding occurred between glycerol and polymers in plasticized films compared to control films. Furthermore, molecular docking was applied to predict the binding interactions betweem AmB, CMC, gelatin, SA and glycerol, which further endorsed the stabilizing effects of glycerol in the complex formation between AmB, CMC, SA, and gelatin. The Findings of the current study demonstrated that this polymeric blend could be used to successfully prepare bioadhesive films with glycerol as a plasticizer.
