Nature Communications (Jul 2021)

Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress

  • Luiza Mendonça,
  • Andrew Howe,
  • James B. Gilchrist,
  • Yuewen Sheng,
  • Dapeng Sun,
  • Michael L. Knight,
  • Laura C. Zanetti-Domingues,
  • Benji Bateman,
  • Anna-Sophia Krebs,
  • Long Chen,
  • Julika Radecke,
  • Vivian D. Li,
  • Tao Ni,
  • Ilias Kounatidis,
  • Mohamed A. Koronfel,
  • Marta Szynkiewicz,
  • Maria Harkiolaki,
  • Marisa L. Martin-Fernandez,
  • William James,
  • Peijun Zhang

DOI
https://doi.org/10.1038/s41467-021-24887-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

Read online

In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-2 infection cycle in the context of whole-cell images.