Molecular Diagnosis of Helicobacter pylori Endosymbiont in Acanthamoeba-Positive Samples in Laboratory Conditions and in the Hospital Environments

Abstract

Background: We aimed to identity Helicobacter pylori endosymbiont in Acanthamoeba-positive samples in natural and laboratory conditions. Methods: Overall, 134 samples were collected from hospital environments. Microscopic and PCR test were used for detection of Acanthamoeba and H. pylori. The real-time PCR method was used to check the active presence of H. pylori within Acanthamoeba under natural conditions from hospital samples and in co-culture laboratory conditions. Results: The rate of contamination of hospital samples with Acanthamoeba was 44.7%. Out of 42 Acanthamoeba PCR-positive samples, 13 isolates (31%) were positive in terms of H. pylori endosymbiont according to sampling location. H. pylori is able to penetrate and enter the Acanthamoeba parasite. Conclusion: H. pylori is able to contaminate Acanthamoeba in natural and laboratory conditions. The presence of pathogenic Acanthamoeba in various hospital environments and the hiding of Helicobacter as an endosymbiont inside it can pose a serious threat to the health of hospitalized patients.

Keywords

• Acanthamoeba
• Helicobacter pylori
• Endosymbiont
• Hospital