Frontiers in Veterinary Science (Dec 2021)
Isolation and Molecular Characteristics of a Novel Recombinant Avian Orthoreovirus From Chickens in China
- Jun Zhang,
- Jun Zhang,
- Jun Zhang,
- Jun Zhang,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Tuofan Li,
- Weikang Wang,
- Weikang Wang,
- Weikang Wang,
- Weikang Wang,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Quan Xie,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao
Affiliations
- Jun Zhang
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jun Zhang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jun Zhang
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Jun Zhang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Tuofan Li
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Tuofan Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Tuofan Li
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Tuofan Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Weikang Wang
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Weikang Wang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Weikang Wang
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Weikang Wang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Quan Xie
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Quan Xie
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Quan Xie
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Quan Xie
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Zhimin Wan
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Aijian Qin
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Aijian Qin
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Aijian Qin
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Aijian Qin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jianqiang Ye
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Ministry of Education Key Laboratory for Avian Preventive Medicine, Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hongxia Shao
- Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2021.771755
- Journal volume & issue
-
Vol. 8
Abstract
In recent years, the emergence of avian orthoreovirus (ARV) has caused significant losses to the poultry industry worldwide. In this study, a novel ARV isolate, designated as AHZJ19, was isolated and identified from domestic chicken with viral arthritis syndrome in China. AHZJ19 can cause typical syncytial cytopathic effect in the chicken hepatocellular carcinoma cell line, LMH. High-throughput sequencing using Illumina technology revealed that the genome size of AHZJ19 is about 23,230 bp, which codes 12 major proteins. Phylogenetic tree analysis found that AHZJ19 was possibly originated from a recombination among Hungarian strains, North American strains, and Chinese strains based on the sequences of the 12 proteins. Notably, the σC protein of AHZJ19 shared only about 50% homology with that of the vaccine strains S1133 and 1733, which also significantly differed from other reported Chinese ARV strains. The isolation and molecular characteristics of AHZJ19 provided novel insights into the molecular epidemiology of ARV and laid the foundation for developing efficient strategies for control of ARV in China.
Keywords
- avian orthoreovirus
- isolation
- genomic characteristics
- phylogenetic analysis
- recombination
- σC protein
