Effects of LPS, Poly (I:C) and <i>Edwardsiella tarda</i> on the Expression Patterns of IL-17 Family Members and Their Receptors in Spotted Sea Bass (<i>Lateolabrax maculatus</i>)
Shuai Wan,
Zhaosheng Sun,
Chang Zhang,
Tingshuang Pan,
Shuya Yuan,
Yuxi Chen,
Jun Zou,
Qian Gao
Affiliations
Shuai Wan
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
Zhaosheng Sun
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
Chang Zhang
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
Tingshuang Pan
Institute of Fisheries Science, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Shuya Yuan
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
Yuxi Chen
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
Jun Zou
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
Qian Gao
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China
In fish, the immune system plays a crucial role in defending against pathogen-induced infections. The interleukin 17 (IL-17) family, which is a well-studied class of cytokines, serves as a key component of the immune response against external pathogens. In this research, four IL-17 ligands (IL-17A/F1, IL-17B, IL-17C and IL-17D) and one receptor (IL-17RB) genes were identified from spotted sea bass (Lateolabrax maculatus). Alignment analysis showed that the C-terminal region of IL-17 ligands in spotted sea bass was highly conserved. The expression of the IL-17 ligand and receptor genes differed in unstimulated tissues. To investigate the impact of various factors on the immune response of spotted sea bass, we assessed the effect of lipopolysaccharide (LPS), polyinosinic–polycytidylic acid [poly (I:C)] and Edwardsiella tarda treatment on the IL-17 ligands and receptor responses. Our results reveal that the expression of the IL-17 ligand and receptor transcripts is modulated by LPS, poly (I:C) and E. tarda, indicating their significant role in the immune system.
