Diterpenoids from the aerial parts of Isodon macrocalyx
Yanling CHANG,
Xiaoqin LIANG,
Yan HUANG,
Liwei PAN,
Ping HOU,
Chenyang REN,
Jun LI
Affiliations
Yanling CHANG
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Xiaoqin LIANG
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Yan HUANG
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Liwei PAN
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Ping HOU
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Chenyang REN
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
Jun LI
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
To study the constituents of Isodon macrocalyx, thirteen diterpenoids were isolated and purified from the aerial parts of I. macrocalyx by means of various column chromatographic techniques, including silica gel, ODS, Sephadex LH-20 and RP-C18 pre-HPLC. The structures of the isolated diterpenoids were determined on the basis of analyses of spectroscopic methods (1H NMR and 13C NMR spectroscopy), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and comparison of their spectroscopic data with previously reported data. The results showed that the diterpenoids were identified as 19-hydroxytotarol (1), macrophynin E (2), inumakoic acid (3), inumakiol D (4), 4β-carboxy-19-nortotarol (5), (-)-lambertic acid (6), 2-oxo-5-fagonene (7), isodoterniofiln B (8), longikaurin E (9), longikaurin A (10), rabdotemin H (11), 16S-dihydrolongikaurin A (12), and ent-3S,16S,17-trihydroxy-kauran-2-one (13). All diterpenoids were isolated from I. macrocalyx for the first time.
