STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers

Frontiers in Genetics. 2018;9 DOI 10.3389/fgene.2018.00087

 

Journal Homepage

Journal Title: Frontiers in Genetics

ISSN: 1664-8021 (Online)

Publisher: Frontiers Media S.A.

LCC Subject Category: Science: Biology (General): Genetics

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB, XML

 

AUTHORS

Loan T. Nguyen (School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia)
Loan T. Nguyen (Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam)
Antonio Reverter (CSIRO Agriculture and Food, Queensland Bioscience Precinct, St. Lucia, QLD, Australia)
Angela Cánovas (Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada)
Bronwyn Venus (Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia)
Stephen T. Anderson (School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia)
Alma Islas-Trejo (Department of Animal Science, University of California, Davis, Davis, CA, United States)
Marina M. Dias (Departamento de Zootecnia, Faculdade de Ciências Agráìrias e Veterináìrias, Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil)
Natalie F. Crawford (Department of Animal Science, Colorado State University, Fort Collins, CO, United States)
Sigrid A. Lehnert (CSIRO Agriculture and Food, Queensland Bioscience Precinct, St. Lucia, QLD, Australia)
Juan F. Medrano (Department of Animal Science, University of California, Davis, Davis, CA, United States)
Milt G. Thomas (Department of Animal Science, Colorado State University, Fort Collins, CO, United States)
Stephen S. Moore (Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia)
Marina R. S. Fortes (School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia)
Marina R. S. Fortes (Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 14 weeks

 

Abstract | Full Text

The liver plays a central role in metabolism and produces important hormones. Hepatic estrogen receptors and the release of insulin-like growth factor 1 (IGF1) are critical links between liver function and the reproductive system. However, the role of liver in pubertal development is not fully understood. To explore this question, we applied transcriptomic analyses to liver samples of pre- and post-pubertal Brahman heifers and identified differentially expressed (DE) genes and genes encoding transcription factors (TFs). Differential expression of genes suggests potential biological mechanisms and pathways linking liver function to puberty. The analyses identified 452 DE genes and 82 TF with significant contribution to differential gene expression by using a regulatory impact factor metric. Brain-derived neurotrophic factor was observed as the most down-regulated gene (P = 0.003) in post-pubertal heifers and we propose this gene influences pubertal development in Brahman heifers. Additionally, co-expression network analysis provided evidence for three TF as key regulators of liver function during pubertal development: the signal transducer and activator of transcription 6, PBX homeobox 2, and polybromo 1. Pathway enrichment analysis identified transforming growth factor-beta and Wnt signaling pathways as significant annotation terms for the list of DE genes and TF in the co-expression network. Molecular information regarding genes and pathways described in this work are important to further our understanding of puberty onset in Brahman heifers.