Antioxidant Effect of Continuous Training with Crocin Consumption on Doxorubicin-Induced Hepatotoxicity in Rats

Abstract

Introduction: It has been reported that doxorubicin can cause liver damage; however, exercise and crocin consumption can improve antioxidant defense.The aim of the present study was to investigate the antioxidant effect of continuous training with crocin consumption on the liver tissue of doxorubicin-poisoned rats. Methods: In this experimental study, 40 rats were selected and divided into five groups of eight subjects, including: 1)unhealthy control(Dox), 2) crocin consumption, 3) continuous training, and 4) continuous training with crocin consumption and 5) healthy control( salin). For eight weeks, groups 1 to 4 received 2 mg / kg of doxorubicin peritoneally seven times each Friday throughout the study period. Groups 1 and 4 received 10 mg / kg of crocin peritoneally per day, and groups 3 and 4 performed five sessions of continuous training per week and group 5 only injected by normal salin 0.9%. Results: Doxorubicin induction had a significant effect on decreasing SOD and CAT as well as increasing MDA ; continuous training and crocin consumption had a significant effect on increasing SOD and CAT in doxorubicin-poisoned rats P˂(0.05), however, continuous training along with crocin consumption had no interactive effect on increasing SOD and CAT in doxorubicin-poisoned rats P˃(0.05); continuous training and crocin consumption had a significant effect on decreasing MDA in doxorubicin-poisoned rats. Also, continuous training along with crocin consumption had interactive effect on decreasing MDA in the liver tissue of doxorubicin-poisoned rats P˂ (0.05). Conclusion: It seems that continuous training along with crocin consumption has an interactive effect on decreasing MDA in the liver tissue of doxorubicin-poisoned rats, however it has no interactive effect on increasing SOD and CAT.

Keywords

• continuous training
• doxorubicin
• crocin
• oxidative enzymes