Obtención de vitroplantas de Escobedia grandiflora (Fam: Orobanchaceae) a partir de semilla

Abstract

Escobedia grandiflora (L.f) Kuntze is an hemiparasite plant, whose propagation is difficult. The aims of this study were: (1) to determine the protocol for E. grandiflora seed disinfection; (2) to evaluate seed germination with 3-Indole Acetic Acid (AIA) and Gibberellic Acid (AG3) application; and (3 to evaluate the in vitro seedling development in two types of gelling with different AIA concentrations. Optimal disinfection was achieved using sodium hypochlorite since 0% of the seedling were contaminated and 100% germinated. Additionally, a better germination was obtained without the use of hormones, also Phytagel gelling favored the seedling growth. Regarding the AIA concentration, the seedlings reached a better length with 10mg/l of AIA, however a greater root length was found without the application of AIA. We conclude that it is possible to obtain E. grandiflora vitroplants from the seed without AIA and AG3 hormones addition. However, it is important to evaluate other hormones and concentrations in order to reduce seedling time in in vitro conditions.

Keywords

• escobedia grandiflora
• in vitro plants; germination
• aia hormone