Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada; Kynon J.M. Benjamin; Anna C. Brandtjen; Ethan Tietze; Samuel J. Allen; Apuã C.M. Paquola; Joel E. Kleinman; Thomas M. Hyde; Jennifer A. Erwin

Stem Cell Research (Jul 2020)

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada,
Kynon J.M. Benjamin,
Anna C. Brandtjen,
Ethan Tietze,
Samuel J. Allen,
Apuã C.M. Paquola,
Joel E. Kleinman,
Thomas M. Hyde,
Jennifer A. Erwin

Affiliations

Tomoyo Sawada: Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA; Corresponding authors: Lieber Institute for Brain Development, Baltimore, Maryland, USA
Kynon J.M. Benjamin: Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Anna C. Brandtjen: Lieber Institute for Brain Development, Baltimore, Maryland, USA
Ethan Tietze: Lieber Institute for Brain Development, Baltimore, Maryland, USA
Samuel J. Allen: Lieber Institute for Brain Development, Baltimore, Maryland, USA
Apuã C.M. Paquola: Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Joel E. Kleinman: Lieber Institute for Brain Development, Baltimore, Maryland, USA
Thomas M. Hyde: Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Jennifer A. Erwin: Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland, USA; Corresponding authors: Lieber Institute for Brain Development, Baltimore, Maryland, USA

Journal volume & issue: Vol. 46
p. 101806

Abstract

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In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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